Core Application Scenarios
1. Protein Quantification: As a key reagent in the BCA protein quantification method, it binds to cuprous ions—produced by the reduction of proteins in an alkaline environment—to form a purple complex with maximum absorbance at 562 nm. By leveraging the direct proportional relationship between absorbance and protein concentration, it accurately determines the protein concentration in unknown samples. With high sensitivity and a wide linear range, it is a mainstream assay reagent in the fields of biochemistry and molecular biology
2. Expanded Research Applications: It can mediate Cu(I)-catalyzed azide-alkyne cycloaddition click chemistry reactions to achieve the bioconjugation of fluorophores or biotin with functionalized proteins. It can also serve as a tool compound for drug discovery research related to the pathological mechanisms of Alzheimer’s disease and antitumor activity.
Standard Storage Requirements
1. Routine Short-Term Storage: Store in a sealed container in a dry environment at 2–8°C, taking precautions against moisture.
2. Long-Term Stable Storage: Recommended to store in a sealed container at -20°C, protected from light; the shelf life in solid form can reach up to 2 years.
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